Reproduction Abstracts

In Cdx2-deficient mouse embryos, Oct-4 and Nanog expression are not restricted to the inner cell mass (ICM), and trophectoderm (TE) development is impaired. These results suggest that Cdx2 regulates Oct-4 and Nanog expression in mouse embryos, and plays a key role in TE development. The objective of this study was to investigate the role of CDX2 in the early development of bovine embryos. We attempted CDX2 downregulation in bovine embryos by short interfering RNA (siRNA), and ...

GLIS1 markedly enhances the generation of iPS cells from both mouse and human fibroblasts. In mouse, GLIS1 is enriched in unfertilized oocytes and 1-cell stage embryos. Therefore, it is possible that GLIS1 plays a critical role in early development through epigenetic regulation. We investigated the role of GLIS1 during the early development of bovine embryos using siRNAs targeted for GLIS1. GLIS1 transcript levels in unfertilized oocytes and early embryos at ...